12 January - 20 June 2016

Method validation for the quantitative analysis of aflatoxins (B1, B2, G1, and G2) and ochratoxin A in processed cereal-based foods

16 December 2015

A team from the University of Istanbul have validated a modified AOAC method to determine aflatoxins and ochratoxins by fluorescence HPLC. The team used a methanol/water extraction followed by purification with an immunoaffinity column prior to analysis.

The validation procedure was carried out on the modified AOAC methods and used Eurachem guidelines for method validation, including parameters such as LOD/LOQ, linearity, accuracy and precision and recovery.

For the aflatoxins the mobile phase used was acetonitrile/methanol/water with wavelengths of 365 and 435nm for excitation and emission. On the other hand the ochratoxins used a methanol/water/acetic acid mobile phase with 333nm and 460nm for the analytical wavelenegths.

Once validated the method was then used to 81 cereal based products available in Turkey such as bread, cakes and flours for the presence of alfatoxins and ochratoxins.

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