Bacterial endotoxin testing

RSSL can screen for the presence of endotoxins in drug products, biologics, medical devices and raw materials to help ensure the safety of your finished product.

 

Endotoxins are an integral component of the outer membrane of the cell wall of Gram-negative bacteria, such as E.coli, Salmonella and Pseudomonas. These are introduced into the environment upon cell death, and also in small amounts as part of normal cell physiological activity. 

 

Endotoxins are exogenous pyrogens and tend to induce a rise in body temperature when introduced into the blood or cerebrospinal fluid. While common effects are fever and nausea, in more extreme cases it can manifest as septic shock. It is therefore critical to test for bacterial endotoxins in your raw materials and finished products, to ensure patient safety.

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    Detection of bacterial endotoxins

    RSSL can screen for the presence of endotoxins in: 

     

     

    To do this, we can employ methods including Limulus Amebocyte Lysate (LAL) testing. LAL is an aqueous extract of the blood cells from the horseshoe crab, containing an enzyme system that causes a coagulation reaction when activated by endotoxins. This reaction is the basis of the LAL test method, which includes kinetic endotoxin testing. 

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    Kinetic endotoxin testing

    The kinetic assay is the most sensitive method for determining endotoxin content. RSSL can provide quantitative endotoxin testing using kinetic turbidimetric or kinetic chromogenic assays. Test samples are mixed with a reagent and measured for an increase in optical density (turbidimetric) or colour change (chromogenic). The rate of change is used to determine the endotoxin levels, which are calculated from a reference standard curve. 

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    Recombinant endotoxin testing

    RSSL can also offer recombinant Cascade Reagent (rCR) testing for endotoxins. This method uses the same cascade of reactions as kinetic assays but is an animal-free alternative to LAL tests. Because of this, there is no risk of glucan interference which can sometimes be seen with kinetic assays. 

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