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HPLC is a widely used chromatographic technique in which a liquid mobile phase is used to separate, identify, and quantify the different components of a sample. The technique relies on pumps to pass pressurised liquid solvents through a column filled with solid absorbent materials. HPLC is commonly used in the analysis of pharmaceuticals, food and beverages, environmental samples, and many other applications. HPLC provides several advantages including high sensitivity and its ability to be applied to different compounds and handle complex mixtures.
UPLC is a more advanced and high-resolution form of HPLC that uses smaller particle sizes in the column packing material, which allows for faster separations and improved sensitivity. This technique is commonly used in the analysis of complex mixtures, such as proteins and peptides.
GC is a technique that uses a gaseous mobile phase to separate the different components of a sample. The sample is vaporised and injected into a column packed with a stationary phase, the components are then separated based on their differential interaction with the stationary phase. RSSL has GCs equipped with Headspace Autosamplers, an automated device that extracts the volatile compounds and injects the extracted vapour into the GC system for separation and analysis. GC is commonly used in the analysis of volatile organic compounds, such as those found in pharmaceutical products, food flavours and beverages, and cosmetics.
Preparative Liquid Chromatography (LC) is a chromatographic technique used to separate, isolate and purify large amounts of a target compound from a complex mixture. Unlike analytical LC, which is used to analyse small amounts of a sample, preparative LC is used to produce large quantities of a purified compound for subsequent use in research or industry. RSSL has tandem Mass Spectrometry (MS/MS) and Nuclear Magnetic Resonance (NMR) capabilities which can be used in conjunction with Prep-HPLC.
A chromatographic technique used to separate and quantify ions based on their charge and affinity for a stationary phase. IC has several important applications that contribute to quality control, regulatory compliance, and research. This technique is suitable for application to a wide range of matrices, of which it is commonly used in the analysis of inorganic and organic ions, such as those found in water samples, pharmaceuticals, and food and beverage products.
Ninhydrin is a chemical compound that reacts with primary amino groups in amino acids and proteins, resulting in a colour change that can be used for detection and quantification. This reaction is commonly utilized in amino acid analysis, particularly in chromatographic techniques like thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Ninhydrin-based amino acid analysis is a widely used technique in biochemistry and protein research, allowing for the accurate quantification and identification of amino acids in complex mixtures, this is crucial for understanding protein composition and structure.